Bibliometric analysis on mercury emissions from coal-fired power plants: a systematic review and future prospect.

Coal-fired power plants (CFPPs) are one of the most significant sources of mercury (Hg) emissions certified by the Minamata Convention, which has attracted much attention in recent years. In this study, we used the Web of Science and CiteSpace to analyze the knowledge structure of this field from 2000 to 2022 and then reviewed it systematically. The field of Hg emissions from coal-fired power plants has developed steadily. The research hotspots can be divided into three categories: (1) emission characterization research focused on speciation changes and emission calculations; (2) emission control research focused on control technologies; (3) environmental impact research focused on environmental pollution and health risk. In conclusion, using an oxygen-rich atmosphere for combustion and installing high-efficiency air pollution control devices (APCDs) helped to reduce the formation of Hg0. The average Hg removal rates of APCDs and modified adsorbents after ultra-low emission retrofit were distributed in the range of 82-93% and 41-100%, respectively. The risk level of Hg in combustion by-products was highest in desulfurization sludge (RAC > 10%) followed by fly ash (10% < RAC < 30%) and desulfurization gypsum (1% < RAC < 10%). Additionally, we found that the implementation of pollution and carbon reduction policies in China had reduced Hg emissions from CFPPs by 45% from 2007 to 2015, increased the efficiency of Hg removal from APCDs to a maximum of 96%, and reduced global transport and health risk of atmospheric Hg. The results conjunctively achieved by CiteSpace, and the literature review will enhance understanding of CFPP Hg emission research and provide new perspectives for future research.

Tracking the flows of Hg, As, Cd, Cr, and Pb in Chinese coal-fired industrial boilers.

Coal-fired industrial boilers (CFIBs) are critical anthropogenic contributors of heavy metals (HMs) because of their high coal consumption and complicated air pollution control facilities (APCDs). This study explored the flows of Hg, As, Cd, Cr, and Pb in CFIBs at regional scale by establishing a boiler-level HMs inflow-outflow inventory. The results indicate that large-capacity CFIBs (≥ 65 t/h) are the leading contributors to HMs inflows. The inflow intensities of HMs in the provinces exhibited three classes of clustering characteristics. Significant regional heterogeneity was characterized by the distribution of HMs inflows and outflows, with higher HMs inflows and outflows in the northern and east-central coastal areas. However, the relatively low synergistic control efficiency of Cd in Northwest China resulted in a higher contribution of waste than inflow. The wastes generated during the operation of CFIBs are the major outflows of HMs. Hg was observed to have the highest outflow of atmospheric emissions owing to its high volatility. In addition, significant differences in the magnitude of HM outflow were identified among the provinces. The application of efficient APCDs contributes significantly to the partitioning of HMs into waste flows, thereby decreasing regional atmospheric emissions.

Silver Mineralized Protein Hydrogel with Intrinsic Cell Proliferation Promotion and Broad-Spectrum Antimicrobial Properties for Accelerated Infected Wound Healing.

The presence of multidrug-resistant bacteria has challenged the clinical treatment of bacterial infection. There is a real need for the development of novel biocompatible materials with broad-spectrum antimicrobial activities. Antimicrobial hydrogels show great potential in infected wound healing but are still being challenged. Herein, broad-spectrum antibacterial and mechanically tunable amyloid-based hydrogels based on self-assembly and local mineralization of silver nanoparticles are reported. The mineralized hydrogels are biocompatible and have the advantages of sustained release of silver, prolonged antimicrobial effect, and improved adhesion capacity. Moreover, the mineralized hydrogels display a significant antimicrobial effect against both Gram-positive and Gram-negative bacteria in cells and mice by inducing membrane damage and reactive oxygen species toxicity in bacteria. In addition, the mineralized hydrogels can rapidly accelerate wound healing by the synergy between their antibacterial activity and intrinsic improvement for cell proliferation and migration. This study provides a modular approach to developing a multifunctional protein hydrogel platform based on biomolecule-coordinated self-assembly for a wide range of biomedical applications.

Review on magnetic adsorbents for removal of elemental mercury from coal combustion flue gas.

Under the influence of human activities, atmospheric mercury (Hg) concentrations have increased by 450% compared with natural levels. In the context of the Minamata Convention on Mercury, which came into effect in August 2017, it is imperative to strengthen Hg emission controls. Existing Air Pollution Control Devices (APCDs) combined with collaborative control technology can effectively remove Hg2+ and Hgp; however, Hg0 removal is substandard. Compared with the catalytic oxidation method, Hg0 removal through adsorbent injection carries the risk of secondary release and is uneconomical. Magnetic adsorbents exhibit excellent recycling and Hg0 recovery performance and have recently attracted the attention of researchers. This review summarizes the existing magnetic materials for Hg0 adsorption and discusses the removal performances and mechanisms of iron, carbon, mineral-based, and magnetosphere materials. The effects of temperature and different flue gas components, including O2, NO, SO2, H2O, and HCl, on the adsorption performance of Hg0 are also summarized. Finally, different regeneration methods are discussed in detail. Although the research and development of magnetic adsorbents has progressed, significant challenges remain regarding their application. This review provides theoretical guidance for the improvement of existing and development of new magnetic adsorbents.

Effect of COVID-19 home confinement on the efficacy of orthokeratology, 0.01% atropine and combined treatment.

PURPOSE: To investigate the effect of COVID-19 home confinement on the efficacy of the interventions for controlling myopia, and to select effective therapies to control myopia during COVID-19 confinement. METHOD: Children (n = 164) aged 8-12 years with spherical equivalent refraction of -1.00 to -6.00 diopters were stratified into two age subgroups and randomly allocated into the control, 0.01% atropine, orthokeratology (ortho-k) and atropine combined ortho-k (ACO) groups. Axial length (AL) was measured at baseline, 6-, 12-, 18- and 24-month visits. The follow-up spanned the period before the COVID-19 outbreak, the period of the home confinement, and the period of the school reopening. Hence, the AL change in different periods was collected and compared. Data analysis was performed following the criteria of intention to treat (ITT). RESULTS: All 164 children were involved in the ITT analysis. Compared to control, all interventions can still reduce the AL elongation during the COVID-19 home confinement period (all p < 0.05). However, the efficacy was compromised: individuals experienced more AL elongation during the COVID-19 home confinement period in the control, 0.01% atropine and ACO groups (all p < 0.05). Interestingly, in the ortho-k group, the difference was insignificant (p = 0.178), and the interaction between the intervention type (control vs. ortho-k) and the confinement severity was significant (p for interaction = 0.041), which is different from the atropine (p for interaction = 0.248) or ACO group (p for interaction = 0.988). These results were stable after being adjusted by other variables based on the multivariable regression model. CONCLUSION: Ortho-k was less affected by the COVID-19 home confinement, which is potentially a better therapy for children in this high-risk environment. Further investigations are warranted to validate this issue.

Structural Characterization of an N-Acetyl Sugar Amidotransferase Involved in the Lipopolysaccharide Biosynthesis in Bacteria.

N-acetyl sugar amidotransferase (NASAT) is involved in the lipopolysaccharide (LPS) biosynthesis pathway that catalyzes the formation of the acetamido moiety (sugar-NC(=NH)CH3) on the O-chain. So far, little is known about its structural and functional properties. Here, we report the crystal structure of an N-acetyl sugar amidotransferase from Legionella pneumophila (LpNASAT) at 2.33 Å resolution. LpNASAT folds into a compact basin-shaped architecture with an unusually wide and open putative substrate-binding pocket and a conserved zinc ion-binding tetracysteine motif. The pocket contains a Rossmann-like fold with a PP-loop, suggesting that the NASAT-catalyzed amidotransfer reaction probably requires the conversion of ATP to AMP and PPi. Our data provide structural insights into the NASAT family of proteins, and allow us to possibly identify its functionally important regions.

Case Study: Impacts of Air-Conditioner Air Supply Strategy on Thermal Environment and Energy Consumption in Offices Using BES-CFD Co-Simulation.

Due to energy constraints and people's increasing requirements for indoor thermal comfort, improving energy efficiency while ensuring thermal comfort has become the focus of research in the design and operation of HVAC systems. This study took office rooms with few people occupying them in Wuhan as the research object. The EnergyPlus-Fluent co-simulation method was used to study the impact of 12 forms of air distribution on the thermal environment and air-conditioner energy consumption. The results indicate that 3 m/s supply air velocity and 45° supply air angle are more suitable for the case model in this study. The EnergyPlus-Fluent co-simulation method used in this paper provides a reference for the study of indoor environments in offices with few people occupying them.

Implantation of hydrogel-liposome nanoplatform inhibits glioblastoma relapse by inducing ferroptosis.

Glioblastoma is acknowledged as the most aggressive cerebral tumor in adults. However, the efficacy of current standard therapy is seriously undermined by drug resistance and suppressive immune microenvironment. Ferroptosis is a recently discovered form of iron-dependent cell death that may have excellent prospect as chemosensitizer. The utilization of ferropotosis inducer Erastin could significantly mediate chemotherapy sensitization of Temozolomide and exert anti-tumor effects in glioblastoma. In this study, a combination of hydrogel-liposome nanoplatform encapsulated with Temozolomide and ferroptosis inducer Erastin was constructed. The αvß3 integrin-binding peptide cyclic RGD was utilized to modify codelivery system to achieve glioblastoma targeting strategy. As biocompatible drug reservoirs, cross-linked GelMA (gelatin methacrylamide) hydrogel and cRGD-coated liposome realized the sustained release of internal contents. In the modified intracranial tumor resection model, GelMA-liposome system achieved slow release of Temozolomide and Erastin in situ for more than 14 d. The results indicated that nanoplatform (T+E@LPs-cRGD+GelMA) improved glioblastoma sensitivity to chemotherapeutic temozolomide and exerted satisfactory anti-tumor effects. It was demonstrated that the induction of ferroptosis could be utilized as a therapeutic strategy to overcome drug resistance. Furthermore, transcriptome sequencing was conducted to reveal the underlying mechanism that the nanoplatform (T+E@LPs-cRGD+GelMA) implicated in. It is suggested that GelMA-liposome system participated in the immune response and immunomodulation of glioblastoma via interferon/PD-L1 pathway. Collectively, this study proposed a potential combinatory therapeutic strategy for glioblastoma treatment.

Functional-oriented, portable brain-computer interface training for hand motor recovery after stroke: a randomized controlled study.

Background: Brain-computer interfaces (BCIs) have been proven to be effective for hand motor recovery after stroke. Facing kinds of dysfunction of the paretic hand, the motor task of BCIs for hand rehabilitation is relatively single, and the operation of many BCI devices is complex for clinical use. Therefore, we proposed a functional-oriented, portable BCI equipment and explored the efficiency of hand motor recovery after a stroke. Materials and methods: Stroke patients were randomly assigned to the BCI group and the control group. The BCI group received BCI-based grasp/open motor training, while the control group received task-oriented guidance training. Both groups received 20 sessions of motor training in 4 weeks, and each session lasted for 30 min. The Fugl-Meyer assessment of the upper limb (FMA-UE) was applied for the assessment of rehabilitation outcomes, and the EEG signals were obtained for processing. Results: The progress of FMA-UE between the BCI group [10.50 (5.75, 16.50)] and the control group [5.00 (4.00, 8.00)] was significantly different (Z = -2.834, P = 0.005). Meanwhile, the FMA-UE of both groups improved significantly (P < 0.001). A total of 24 patients in the BCI group achieved the minimal clinically important difference (MCID) of FMA-UE with an effective rate of 80%, and 16 in the control group achieved the MCID, with an effective rate of 51.6%. The lateral index of the open task in the BCI group was significantly decreased (Z = -2.704, P = 0.007). The average BCI accuracy for 24 stroke patients in 20 sessions was 70.7%, which was improved by 5.0% in the final session compared with the first session. Conclusion: Targeted hand movement and two motor task modes, namely grasp and open, to be applied in a BCI design may be suitable in stroke patients with hand dysfunction. The functional-oriented, portable BCI training can promote hand recovery after a stroke, and it is expected to be widely used in clinical practice. The lateral index change of inter-hemispheric balance may be the mechanism of motor recovery. Trial registration number: ChiCTR2100044492.

The bacterial effector SidN/Lpg1083 promotes cell death by targeting Lamin-B2.

To facilitate survival, replication, and dissemination, the intracellular pathogen Legionella pneumophila relies on its unique type IVB secretion system (T4SS) to deliver over 330 effectors to hijack host cell pathways in a spatiotemporal manner. The effectors and their host targets are largely unexplored due to their low sequence identity to the known proteins and functional redundancy. The T4SS effector SidN (Lpg1083) is secreted into host cells during the late infection period. However, to the best of our knowledge, the molecular characterization of SidN has not been studied. Herein, we identified SidN as a nuclear envelope-localized effector. Its structure adopts a novel fold, and the N-terminal domain is crucial for its specific subcellular localization. Furthermore, we found that SidN is transported by eukaryotic karyopherin Importin-13 into the nucleus, where it attaches to the N-terminal region of Lamin-B2 to interfere with the integrity of the nuclear envelope, causing nuclear membrane disruption and eventually cell death. Our work provides new insights into the structure and function of an L. pneumophila effector protein, and suggests a potential strategy utilized by the pathogen to promote host cell death and then escape from the host for secondary infection.

Fear-of-intimacy-mediated zinc transport is required for Drosophila fat body endoreplication.

BACKGROUND: Endoreplication is involved in the development and function of many organs, the pathologic process of several diseases. However, the metabolic underpinnings and regulation of endoreplication have yet to be well clarified. RESULTS: Here, we showed that a zinc transporter fear-of-intimacy (foi) is necessary for Drosophila fat body endoreplication. foi knockdown in the fat body led to fat body cell nuclei failure to attain standard size, decreased fat body size and pupal lethality. These phenotypes could be modulated by either altered expression of genes involved in zinc metabolism or intervention of dietary zinc levels. Further studies indicated that the intracellular depletion of zinc caused by foi knockdown results in oxidative stress, which activates the ROS-JNK signaling pathway, and then inhibits the expression of Myc, which is required for tissue endoreplication and larval growth in Drosophila. CONCLUSIONS: Our results indicated that FOI is critical in coordinating fat body endoreplication and larval growth in Drosophila. Our study provides a novel insight into the relationship between zinc and endoreplication in insects and may provide a reference for relevant mammalian studies.

Robust flexural performance and fracture behavior of TiO2 decorated densified bamboo as sustainable structural materials.

High-performance, fast-growing natural materials with sustainable and functional features currently arouse significant attention. Here, facile processing, involving delignification, in situ hydrothermal synthesis of TiO2 and pressure densification, is employed to transform natural bamboo into a high-performance structural material. The resulting TiO2-decorated densified bamboo exhibits high flexural strength and elastic stiffness, with both properties more than double that of natural bamboo. Real-time acoustic emission reveals the key role of the TiO2 nanoparticles in enhancing the flexural properties. The introduction of nanoscale TiO2 is found to markedly increase the degree of oxidation and the formation of hydrogen bonds in bamboo materials, leading to extensive interfacial failure between the microfibers, a micro-fibrillation process that results in substantial energy consumption and high fracture resistance. This work furthers the strategy of the synthetic reinforcement of fast-growing natural materials, which could lead to the expanded applications of sustainable materials for high-performance structural applications.

Refined assessment and decomposition analysis of carbon emissions in high-energy intensive industrial sectors in China.

Carbon emissions from high-energy intensive industrial sectors are the focus of this study due to the huge energy consumption of these sectors. A refined carbon emission inventory of Chinese high-energy intensive industrial sectors in 2020 was first developed at the point source level. The results showed that coal-fired power plants (CFPPs) were the leading contributors to carbon emissions, followed by iron and steel smelting (ISS) and cement production (CMP). Provinces with high carbon emission intensity were mainly concentrated in the north and northeast coasts, while exhibiting a developed economic level and a concentration of heavy industries. Additionally, the growth in China's industrial carbon emissions from 1995 to 2020 can be divided into three phases. The largest decrease in emission intensity was observed in Central, Southwest, North, and East China. Furthermore, the economic structure remained the dominant driver of carbon emissions from the 10th to 13th Five-Year Plan (FYP), playing a positive promotional role. The contribution of economic structure, energy intensity, and energy structure to carbon emissions varied substantially by region and period. With the proposal of sustainable development and energy conservation in China, the influence of economic structure on the carbon emissions of industrial sectors has gradually weakened since the 11th FYP. The reduction in industrial carbon emissions in China under three scenarios could reach up to 46.6 % from 2030 to 2050. The results indicate that industrial carbon emission control in China needs to be integrated into the refined control pathway for conventional air pollutants, considering the spatial variability of industrial carbon emissions in China.

Application of loofah and insects in a bio-trickling filter to relieve clogging.

Bio-trickling filters (BTFs) use an inert filler to purify pollutants making them prone to clogging due to bacterial accumulation. To investigate the performance of a non-inert filler in BTF and its cooperation with insects to relieve clogging, a vertical BTF was constructed with a loofah/Pall ring/polydimethylsiloxane composite filler and selected bacteria to purify toluene. The BTF was started up within 17 d and restarted within 3 d after starvation for 12-16 d. Its average removal efficiency was >90% at steady state. The maximum elimination capacity of 86.4 g·(m3·h)-1 was obtained at a volume capacity of 96.2 g·(m3·h)-1. The introduction of holometabolous insects (Clogmia albipunctata) rapidly removed the biofilm and accelerated the degradation of the loofah, which alleviated clogging. Furthermore, confocal laser scanning microscope (CLSM) observations showed that the biofilm polysaccharides were difficult to remove, while lipids were readily lost. Analysis of microbial diversity over time and space revealed that the dominant bacterium, Comamonas, was replaced by diverse microflora with no obvious dominant genus. Insect introduction and loofah migration had little effect on the evolution of microflora. This study provides a promising approach to operating BTFs with less clogging.

Distribution, chemical fractionation, and potential environmental risks of Hg, Cr, Cd, Pb, and As in wastes from ultra-low emission coal-fired industrial boilers in China.

This study conducted a comprehensive investigation of the distribution, chemical fractionation, and potential environmental risks of Hg, Cd, Cr, Pb, and As in waste based on new data from five ultra-low emission (ULE) coal-fired industrial boilers (CFIBs). The results showed that fly ash was enriched with Cd, Pb, As, and Hg, while its Cr contents were not invariably higher than those of slag. Fly ash was the predominant output flow for Hg, Cd, Cr, Pb, and As in the tested ULE boilers, with higher proportions of HTEs in the fly ash and lower proportions of HTEs in the flue gas than in the non-ULE boilers. The average proportions of residual Hg, Cd, Cr, Pb, and As in wastes revealed the following order: slag > fly ash > flue gas desulfurization (FGD) by-products. The potential environmental risks of Hg, Cd, Cr, Pb, and As in the fly ash, slag, and FGD by-products of CFIBs at the county level in the Beijing-Tianjin-Hebei Air Pollution Transmission Channel Cities ("2 +26 cities") region showed spatial heterogeneity. It is predicted that the potential release of Pb, Cr, and Cd in the fly ash would increase and that of the FGD by-products would decrease after the implementation of the ULE retrofitting of all CFIBs.

N6-Methyladenosine-modified lncRNA LINREP promotes Glioblastoma progression by recruiting the PTBP1/HuR complex.

Glioblastoma multiforme (GBM) is acknowledged as the most aggressive primary brain tumor in adults. It is typically characterized by the high heterogeneity which corresponds to extensive genetic mutations and complex alternative splicing (AS) profiles. Known as a major repressive splicing factor in AS, polypyrimidine tract-binding protein 1 (PTBP1) is involved in the exon skipping events of multiple precursor mRNAs (pre-mRNAs) in GBM. However, precise mechanisms that modulate the expression and activity of PTBP1 remain to be elucidated. In present study, we provided evidences for the role of a long intergenic noncoding RNA (LINREP) implicated in the regulation of PTBP1-induced AS. LINREP interacted with PTBP1 and human antigen R (HuR, ELAVL1) protein complex and protected PTBP1 from the ubiquitin-proteasome degradation. Consequently, a broad spectrum of PTBP1-induced spliced variants was generated by exon skipping, especially for the skipping of reticulon 4 (RTN4) exon 3. Interestingly, LINREP also promoted the dissociation of nuclear UPF1 from PTBP1, which increased the binding of PTBP1 to RTN4 transcripts, thus enhancing the skipping of RTN4 exon 3 to some extent. Besides, HuR recruitment was essential for the stabilization of LINREP via a manner dependent on N6-methyladenosine (m6A) formation and identification. Taken together, our results demonstrated the functional significance of LINREP in human GBM for its dual regulation of PTBP1-induced AS and its m6A modification modality, implicating that HuR/LINREP/PTBP1 axis might serve as a potential therapeutic target for GBM.

A modelling method for large-scale open spaces orientated toward coordinated control of multiple air-terminal units.

The temperature distribution is always assumed to be homogeneous in a traditional single-input-single-output (SISO) air conditioning control strategy. However, the airflow inside is more complicated and unpredictable. This study proposes a zonal temperature control strategy with a thermal coupling effect integrated for air-conditioned large-scale open spaces. The target space was split into several subzones based on the minimum controllable air terminal units in the proposed method, and each zone can be controlled to its own set-point while considering the thermal coupling effect from its adjacent zones. A numerical method resorting to computational fluid dynamics was presented to obtain the heat transfer coefficients (HTCs) under different air supply scenarios. The relationship between heat transfer coefficient and zonal temperature difference was linearized. Thus, currently available zonal models in popular software can be used to simulate the dynamic response of temperatures in large-scale indoor open spaces. Case studies showed that the introduction of HTCs across the adjacent zones was capable of enhancing the precision of temperature control of large-scale open spaces. It could satisfy the temperature requirements of different zones, improve thermal comfort and at least 11% of energy saving can be achieved by comparing with the conventional control strategy. Electronic Supplementary Material ESM: The Appendix is available in the online version of this article at 10.1007/s12273-022-0942-8.

The expression of Catsup in escort cells affects Drosophila ovarian stem cell niche establishment and germline stem cells self-renewal via Notch signaling.

Stem cell niche provides extrinsic signals to maintain stem cell renewal or initiate cell differentiation. Drosophila niche is composed of somatic terminal filament cells, cap cells and escort cells. However, the underlying mechanism for the development of stem cell niche remains largely unclear. Here we found that the expression of a zinc transporter Catsup is essential for ovary morphogenesis. Catsup knockdown in escort cells results in defects of niche establishment and germline stem cells self-renewal. These defects could be modified by altered expression of genes involved in zinc metabolism or intervention of dietary zinc levels. Further studies indicated that Catsup RNAi affected adult ovary morphogenesis by suppressing Notch signaling. Lastly, we demonstrated that the defects of Catsup RNAi could be restored by overexpression of heat shock cognate protein 70 (Hsc70). These findings expand our understanding of the mechanisms controlling adult oogenesis and niche establishment in Drosophila.

The Risk of Postpolypectomy Bleeding in Patients Receiving Direct Oral Anticoagulants compared to Warfarin or Nonanticoagulation: A Systematic Review with Meta-Analysis.

AIM: The aim of our systematic review and meta-analysis was to assess the risk of postpolypectomy bleeding (PPB) in patients exposed to direct oral anticoagulants (DOACs). METHODS: A systematic search was conducted by searching the PubMed, Embase, and Cochrane Library databases using the following search terms: "(nonvitamin K antagonist oral anticoagulants or NOAC or apixaban or dabigatran or rivaroxaban or edoxaban or DOAC or direct oral anticoagulants) and polypectomy". Studies evaluating the association between DOACs and PPB were identified. RESULTS: The bibliographical search yielded 103 studies. Twelve studies involving 621,279 participants were ultimately included (11 cohort studies, of which 10 were retrospective, and a randomized controlled trial.). Pooled estimates revealed a higher risk of PPB among patients using DOACs than among those without anticoagulation (odds ratio [OR]: 6.170, 95% confidence interval [CI]: 3.079 to 12.363). The same result occurred when DOACs were stopped 24 hours before polypectomy (OR: 8.66, 95% CI: 4.588 to 16.348). No significant difference was noted between overall DOACs and warfarin (OR 0.826, 95% CI 0.583 to 1.172), while for subgroups, dabigatran showed a lower PPB rate than warfarin (OR: 0.582, 95% CI: 0.340 to 0.994). CONCLUSIONS: DOACs can significantly raise the risk of PPB, even with 24-hour withdrawal before polypectomy. In addition, a lower risk of PPB was detected for dabigatran than for warfarin.

Circadian clock disruption aggravates alcohol liver disease in an acute mouse model.

Circadian rhythms are important for organisms to adapt to the environment and maintain homeostasis. Disruptions of circadian rhythms contribute to the occurrence, progression, and exacerbation of diseases, such as cancer, psychiatric disorders, and metabolic disorders. Alcohol-induced liver disease (ALD) is one of the most prevalent liver diseases. Disruptions of the circadian clock enhance the ALD symptoms using chronic mice models or genetic manipulated mice. However, chronic models are time consuming and clock gene deletions interfere with metabolisms. Here, we report that constant light (LL) condition significantly disrupted the circadian clock in an acute ALD model, resulting in aggravated ALD phenotypes in wild type mice. Comparative transcriptome analysis revealed that the alcohol feeding affected the circadian pathway, as well as metabolic pathways. The acute alcohol feeding plus the LL condition further interfered with metabolic pathways and dysregulated canonical circadian gene expressions. These findings support the idea that disrupting the circadian clock could provide an improved ALD mouse model for further applications, such as facilitating identification of potential therapeutic targets for the prevention and treatment of ALD.Abbreviations: ALD, alcohol-induced liver disease; LD, 12 h light _ 12 h dark; LL, constant light; HF, high-fat liquid control diet; ETH, ethanol-containing diet; NIAAA, National Institute on Alcohol Abuse and Alcoholism; TTFLs, transcription-translation feedback loops; FDA, US Foods and Drug Administration; NAFLD, non-alcoholic fatty liver disease; RER, respiratory exchange rate; DEGs, differentially expressed genes; H&E, haematoxylin and eosin; ALT, alanine transaminase; AST, aspartate transaminase; TG, triglycerides.